Immunoprecipitation Strategies to Isolate RIPK1/RIPK3 Complexes in Mouse Macrophages
نویسندگان
چکیده
منابع مشابه
Nanomedicine Strategies to Target Tumor-Associated Macrophages
In recent years, the influence of the tumor microenvironment (TME) on cancer progression has been better understood. Macrophages, one of the most important cell types in the TME, exist in different subtypes, each of which has a different function. While classically activated M1 macrophages are involved in inflammatory and malignant processes, activated M2 macrophages are more involved in the wo...
متن کاملIdentification of protein complexes from co-immunoprecipitation data
MOTIVATION Advanced technologies are producing large-scale protein-protein interaction data at an ever increasing pace. A fundamental challenge in analyzing these data is the inference of protein machineries. Previous methods for detecting protein complexes have been mainly based on analyzing binary protein-protein interaction data, ignoring the more involved co-complex relations obtained from ...
متن کاملMouse Macrophages
synthesized from L-arginine (L-Arg) by NO synthase (NOS) ( 1), It has been reported to mediate various physiological events, including defense reactions of the body, and increases in its production lead to the development of various diseases (2), It can regulate blood vessel tone in vascular systems as a biological mediator, and is an important host defense effector in the immune system. On the...
متن کاملChromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction. Such regulation requires the binding of signal-sensitive transcription factors (TFs) that recruit chromatin-modifying enzymes...
متن کاملBioengineering strategies to generate artificial protein complexes.
For many applications, increasing synergy between distinct proteins through organization is important for the specificity, regulation, and overall reaction efficiency. Although there are many examples of protein complexes in nature, a generalized method to create these complexes remains elusive. Many conventional techniques such as random chemical conjugation, physical adsorption onto surfaces,...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Protocols
سال: 2021
ISSN: 2691-1299,2691-1299
DOI: 10.1002/cpz1.156